牦牛Fas和FasL基因克隆及其在主要组织器官中的表达

doi:10.11843/j.issn.0366-6964.2015.02.008

Abstract

Abstract:

To further understand the function of Fas/ FasL system，the Fas and FasL genes of yak were cloned by RT-PCR method and their expressions were analyzed by combination of RT-PCR and immunohistochemistry.A complete cDNA sequences in 2 605 bp of Fas gene and 1 572 bp of FasL gene of yak was obtained.These sequences were highly homology with cattle.Fas and FasL mRNAs were co-expressed in all analyzed tissues except heart which expressed Fas mRNA alone.The two genes strongly co-expressed in liver，spleen，kidney，lymphonodus，thymus and testis.A cytoplasmic form of FasL protein was detected in several cell types of various organs.Fas and FasL were strongly co-expressed in lymphoid organs and immune privilege sites，they were also strongly co-expressed in other tissues like kidney and liver.The results suggested that Fas/FasL system was more complicated.

CLC Number:

S823.8⁺5.2

FAN Jiang-feng，CHEN Peng，CUI Yan，YU Si-jiu. Cloning，Expression，and Localization of Fas and FasL Genes in Yaks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 228-238.

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References

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Cloning，Expression，and Localization of Fas and FasL Genes in Yaks

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